Genotoxicity in the oral cells of older people from a Brazilian rural area: a population-based study

Abstract The purpose of this population-based, observational, and cross-sectional study was to evaluate alterations in the oral cells of a population of older people from a Brazilian rural area, using the micronucleus technique to investigate possible associated genotoxic factors. A questionnaire was applied and clinical examination and collection of oral mucosal cells were performed for all older people (≥ 60 years) from a town in southern Brazil. Demographic and socioeconomic variables, deleterious habits (drinking and tobacco use), presence of gastro-oesophageal reflux disease (GERD), and the use of proton pump inhibitors (PPIs) were considered the exposure variables, whereas metanuclear changes (MCs) and the prevalence of cell micronuclei (MN) were considered outcomes. Out of 489 older people, 447 were included in the study, among whom 50.8% were men with a mean age of 70.9 years and 83.9% had a monthly family income greater than US$ 500.00. GERD symptoms were present in 36.2% of the individuals, and 29.1% used PPIs daily, 53.3% consumed alcoholic beverages, and 46.7% used tobacco. The analysis of 1,000 oral mucosal cells per subject showed a MN frequency of 0-2 per individual, and MCs were detected with an average of 15 units per individual (median = 11 per individual). Poisson regression did not show statistical association between the exposure variables and the outcomes (presence of MN and MCs), except for the use of PPIs, which was a protective factor for the prevalence of MN [PR 0.6 (CI 0.3-0,9)]. Age, sex, family income, tobacco use and drinking, and GERD were not associated with the number of MN and MCs in oral mucosal cells of the investigated older people.

Pain factors related to dental implant surgery: A 7-day observational clinical study

This article aims to associate patient-reported pain intensity in the immediate seven days after dental implant surgery with surgical factors, sex and patient age. The sample was composed of 108 patients from a dental school in southern Brazilian, between 2018 and 2020. The variables torque, pre- and postoperative medication, healing of first and second intention, gender, age, number of quadrants, number of implants and type of surgery were related to the outcome pain. Pain was reported every day by the patient until the seventh day after surgery, using a visual analogic scale (VAS). Univariate Poisson regression models were used to assess the relationship among pain and the factors. Rate ratios were obtained with 95% of confidence intervals. Overall pain was reported as moderate/intense (VAS: 3-10) by 30.56% of patients, mild pain (VAS: 1-3) by 55.56%, and no pain symptoms were reported by 13.89% of patients. Individuals which the implants were installed with high torques (50-80 N) showed more pain (p=0.03) compared to patients which the implants were installed with regular torque (30-45 N). The other factors evaluated were not significant. The torque was the most relevant factor related to pain among the evaluated by the study. High torque generates more postoperative pain than lower torque. The factors gender, age, number of operated quadrants, number of installed implants, type of surgery and pre and postoperative analgesic did not interfere in the patient's pain during the first week after surgery.

Are Stress Associated with Temporomandibular Dysfunction? A Cross-Sectional Study / O Estresse Está Associado à Disfunção Temporomandibular? Um Estudo Transversal

Temporomandibular disorders are frequent in different segments of the population and harm the quality of life of individuals. The present sudy aimed to investigate the association between stress and temporomandibular dysfunction (TMD) in university students in Brazil. All incoming students at the Federal University of Pelotas were invited to participate in this cross-sectional study. A self-administered questionnaire was used with socioeconomic and oral health variables. TMD Fonseca questionnaire "Fonseca's anamnestic index" was used to identify the severity of symptoms of temporomandibular dysfunction. The same was elaborated in the form of Helkimo's anamnestic index. Stress was measured using a modified version of the Perceived Stress Scale and categorized into quartiles. Logistic multivariable regression models were used to analyze the associations of interest controlling for possible confounding variables. A backward stepwise procedure was used to select variables that should be kept in the final model. A total of 2,089 students answered the questionnaires and 82 (3.9%) were classified with the presence of TMD. Adjusted logistic regression shows that chance of presenting temporomandibular dysfunction was higher (OR=2.43; 95%CI=1.04-5.65) when the stress level increased. In conclusion, the mild degree of temporomandibular dysfunction was the most prevalent. Stress was associated with individuals with a higher prevalence of temporomandibular dysfunction. (AU)

As disfunções temporomandibulares são frequentes em diferentes segmentos da população e prejudicam a qualidade de vida dos indivíduos. O presente estudo teve como objetivo investigar a associação entre estresse e disfunção temporomandibular (DTM) em estudantes universitários no Brasil. Todos os alunos ingressantes da Universidade Federal de Pelotas foram convidados a participar deste estudo transversal. Foi utilizado um questionário autoaplicável com variáveis ​​socioeconômicas e de saúde bucal. O questionário TMD Fonseca "Índice anamnésico de Fonseca" foi utilizado para identificar a gravidade dos sintomas da disfunção temporomandibular. O mesmo foi elaborado na forma de índice anamnésico de Helkimo. O estresse foi medido usando uma versão modificada da Perceived Stress Scale e categorizado em quartis. Modelos de regressão logística multivariada foram usados ​​para analisar as associações de interesse controlando possíveis variáveis ​​de confusão. Um procedimento de backward stepwise foi usado para selecionar as variáveis ​​que deveriam ser mantidas no modelo final. Um total de 2.089 alunos responderam aos questionários e 82 (3,9%) foram classificados com presença de DTM. A regressão logística ajustada mostra que a chance de apresentar disfunção temporomandibular foi maior (OR=2,43; IC95%=1,04-5,65) quando o nível de estresse aumentou. Em conclusão, o grau leve de disfunção temporomandibular foi o mais prevalente. O estresse foi associado a indivíduos com maior prevalência de disfunção temporomandibular. (AU)

Are stress and symptoms of depression associated with halitosis? A cross-sectional study

Mood disorders such as stress and depression can promote alterations of several hormones Aim: The present study aimed to evaluate if symptoms of depression and stress are associated with halitosis. The hypothesis is that halitosis is associated with stress and depression symptoms. Methods: All university students' entrants in the Federal University of Pelotas (UFPel) were invited to participate. Self-reported halitosis was measured using a visual analog scale. Students were also asked about the presence of halitosis perceived by close individuals. The stress level was measured using a modified version of the Perceived Stress Scale and symptoms of depression were screened by Patient Health Questionnaire-2. Poisson Regression Models were performed. Results: Of a total of 2,058 students who participated in the study, only 16% of them have reported not having any degree of halitosis and 17.6% were informed of closed individuals having halitosis. After adjustments, stress and depression symptoms remained associated with halitosis selfreported. Individuals from the higher stressed quartile presented higher scores of halitosis (RR 1.37 CI95% [1.24­1.53]). Students who presented depressive symptoms showed higher scores of self-perceived halitosis (RR 1.20 CI95% [1.10­1.32]). When the association of stress and halitosis reported by close individuals was tested, symptoms of depression lost the association in the adjusted model, while individuals from the highest quartile from stress remained associated (PR 1.36 CI95% [1.02­1.81]). Conclusion: Halitosis was associated with stress even after control for oral health and socioeconomic variables

Prospective analysis of craniofacial soccer incidents during FIFA competitions: an observational study

Abstract The aim of this prospective epidemiological study was to evaluate the occurrence of incidents involving the craniofacial region of soccer players during three official FIFA competitions. The craniofacial incidents were identified by video analysis of all 144 matches of two FIFA World Cups (2014/2018) and the 2017 FIFA Confederations Cup. Data collection included incident type, incident severity, site affected, incident cause and referee decision. The multivariate Poisson regression model was performed to analyze the associations between covariates. A total of 238 incidents were observed in the craniofacial region (1.6 incidents/match), representing a rate of 48.5 incidents per 1000 hours. At least 80.6% of the matches presented at least one incident, and, in more than 60%, the referee's decision was no foul. According to severity, 26.8% of the incidents were classified as having mild or high severity. Incidents involving lacerations or fracture presented higher severity compared with hits (IRR 3.45[95%CI: 1.89-6.30]). Head-to-head impacts showed an incidence of severe incidents twice as high as those involving upper extremities (IRR 2.01[95%CI:1.07-3.76]). A high number of craniofacial incidents were observed in the last FIFA competitions. Head-to-head impacts and lacerations or fractures were associated with higher incident severity.

Skin color affect the replacement of amalgam for composite in posterior restorations: a birth-cohort study

Abstract The aim of present study was to estimate the occurrence and associated factors for replacement of amalgam posterior restorations. A representative sample of all 5,914 births from the 1982 in Pelotas birth cohort study was prospectively investigated, and the posterior restorations were assessed at 24 (n = 720) and 31 years of age (n = 539). Individual-level variables, i.e., demographic characteristics, socio-economic factors, oral health conditions and use of dental services, were collected from different waves of the cohort. Tooth-level variables included dental group, estimated time in mouth of each amalgam restoration, and number of restored dental surfaces. Thus, 246 individuals presented 718 amalgam restorations at 24 years of age. After 7 seven years of follow-up, 18.9% of these restorations had been replaced with composite resins. Multilevel Poisson regression models showed that, compared to white individuals, blacks presented a lower risk of replacement of amalgam restorations for composite resins (IRR - 0.39 [0.16-0.95]). Individuals with high educational level at age 31 showed an increased likelihood of replacement of amalgam restorations. Therefore, skin color affects the replacement of amalgam for composite resin in posterior restorations.

Methods and logistics of an oral health cohort of university students from Pelotas, a Brazilian Southern city

Aim: This paper aimed to describe the methodological aspects in the baseline of university students' cohort in a public university in southern Brazil. Methods: A multidisciplinary study was carried out assessing the psychosocial and oral health conditions of university students at the Federal University of Pelotas (UFPel). All new university students who have entered in in UFPel in the first semester of 2016 year were invited to participate. Two self-administered questionnaires were applied in classrooms. Descriptive analysis was performed using Stata 14.0. Results: A total of 3,237 students were eligible. Of these, 2,089 (64.5%) signed the consent form to participate of the study and answered the general questionnaires, and 2,052 (63.4%) students answered the confidential questionnaire. Refuses represented only 1.4% (n=29) of the total sample. Overall, most of the sample was female (52.2%), with age ranging from 18 to 24 years (66.1%), with white skin color (74.1%) and with mothers with educational level at university course (32.4%). A total, 62% reported a family income between 1001.00 and 5000.00 BRL, while only 16.3% reported being from low-income family (≤ 1000.00 BRL). The UFPel offers 63 classroom-based courses classified in nine areas of knowledge. The Applied Social Sciences area comprised 19% of the sample followed by the Linguistics, Letters and Arts (17%) area and the Health sciences area (13%). Conclusion: To the knowledge of researchers, this was the first cohort study of university students in the South of Brazil including more than two thousand participants. This paper described strategies adopted to optimize and to allow data collection in the baseline and in the next follow-ups

Desire of university students for esthetic treatment and tooth bleaching: a cross-sectional study

Aim: The aim of this study was to investigate the prevalence of the desire of university students for esthetic treatment and tooth bleaching, and associated factors. Methods: A cross-sectional study was performed in 2016 with first-semester university students in Pelotas. Data was collected via a self-administered questionnaire including demographic, socioeconomic and psychosocial characteristics. Oral Impact on Daily Performance (OIDP) was assessed and Poisson regression models were used to evaluate the association between the following outcomes: prevalence of desire for esthetic treatment and the tooth bleaching, and exposure variables. A p-value of ≤ 0.05 was considered significant. Results: A total of 2,058 students participated in the study. Refusals represented a mere 1.4% of the total sample. Of the individuals interviewed, 16.1% underwent tooth bleaching and 74.4% reported a desire for esthetic dental treatment. Models showed students with higher family income and with the intention to use private dental services had 65% and 47% higher prevalence of tooth bleaching, respectively. Individuals satisfied with dental color and appearance exhibited a higher prevalence of tooth bleaching, as did those who smoked. On the other hand, students satisfied with dental color (PR= 0.77, 95% CI: 0.73-0.80) and with their dental appearance (PR= 0.82, 95% CI 0.78-0.87) demonstrated less desire to undergo esthetic dental treatment. Moreover, individuals who suffered some impact in OIDP reported a greater desire for esthetic treatment (PR= 1.07, 95% CI: 1.02-1.13). Conclusion: General and psychosocial characteristics were associated with the desire for esthetic treatment and tooth bleaching in university students

Should my composite restorations last forever? Why are they failing?

Abstract Composites resins have become the first choice for direct anterior and posterior restorations. The great popularity is related to their esthetic appearance and reduced need of sound tissue removal as compared with former treatments. Several studies have demonstrated that composite restorations may last long in clinical service. In this review we discuss the factors playing a role on the long-term longevity. Composite restorations have demonstrated a good clinical performance with annual failure rates varying from 1% to 3% in posterior teeth and 1% to 5% in anterior teeth. Factors related to the patients such as caries risk and occlusal stress risk, in addition to socioeconomic factors, may affect the survival significantly. Characteristics of the clinical operators, particularly their decision making when it comes to observing or approaching an existing restoration, are decisive for longevity. Cavity features such as the number of restored walls, composite volume, and presence of endodontic treatment are of major importance and may dictate the service time of the restorative approach. The choice of restorative composite seems to have a minor effect on longevity provided that appropriate technical procedures are used. The main reasons for failure in posterior teeth are secondary caries and fracture (restoration or tooth/restoration), while in anterior teeth esthetic concerns are the main reasons leading to restoration failures. Composite resin restorations can be considered a reliable treatment as long as both the professional and the patient are aware of the factors involved in restoration failures.

Estudo dos traumatismos alvéolo-dentários em pacientes atendidos em um Setor de Cirurgia e Traumatologia Buco-Maxilo-Facial / Study of dentoalveolar trauma in patients treated at an Oral and Maxillofacial Surgery

Objetivo: Avaliar o perfil epidemiológico dos pacientes que sofreram traumatismos alvéolo-dentários em dentes permanentes, identificando também o tipo de trauma, os dentes mais atingidos e as causas mais frequentes. Métodos: A amostra foi constituída por todos os prontuários de pacientes atendidos em cinco anos, no Departamento de Cirurgia da Faculdade de Odontologia de Pelotas, totalizando 308 casos e 793 dentes traumatizados. Os dados obtidos foram tabulados em uma planilha e analisados estatisticamente. Resultados: A faixa etária prevalente foi dos 13 aos 19 anos (32,7%); 74,6% dos traumatismos ocorreram entre pessoas do sexo masculino; a causa mais comum foram os acidentes ciclísticos (23,3%); os tipos de trauma mais frequentes foram a avulsão (17,2%) e a fratura coronária nãocomplicada(17,2%) e os dentes mais acometidos foram os incisivos centrais superiores (66,7%).Conclusão: Foi possível concluir que, nesta pesquisa, a distribuição dos traumatismos foi muito similar a maioria dos estudos publicados, no entanto,em relação à variável causa constatou-se algumas características loco-regionais, reforçando a importância de estudos epidemiológicos para o desenvolvimento de condutas preventivas e terapêuticas específicas para os serviços de saúde.

Objetive: The present study aimed to evaluate the epidemiological profile of patients with traumatic dental injuries in permanent teeth, as well as to identify the type of trauma, the teeth most affected and the most frequent causes of dentoalveolar trauma. Methods: The sample comprised all of the dental records of patients treated at the Oral and Maxillofacial Surgery Department at the Faculty of Dentistryat the Federal University of Pelotas, in the Brazilian state of Rio Grande do Sul, over a five-year period, totaling 308 cases and 793 teeth with traumatic injury. The data were evaluated statistically. Results: The results obtained demonstrated that the most prevalent age group was 13-19 (32.7%); males were affected in 74.6% of injuries; the most common etiology was cycling accidents (23.3%); the most frequent types of trauma were avulsion (17.2%) and non-complicated crown fracture (17.2%); and the most affected teeth were the maxillary central incisors (66.7%). It was concluded that the distribution of trauma injuries, in this paper, is very similar to that presented in most of the published studies. However, with regard to the etiology variable,some local/regional characteristics were found, reinforcing the importance of epidemiological studies for the development of preventive and therapeutic methods specific to the health service.

Survey on the occurrence of dental trauma and preventive strategies among Brazilian professional soccer players

OBJECTIVES: The aims of this study were to verify the occurrence of dental injuries in professional Brazilian soccer players, the level of knowledge of the teams' medical departments about mouthguards, and the conducts adopted in cases of dental trauma during the match. MATERIAL AND METHODS: Closed questionnaires were sent to the physicians in charge of the medical departments of the 40 teams enrolled in the first and second divisions of the Brazilian professional soccer league in 2007. The data obtained were subjected to descriptive analysis to determine absolute and relative frequencies of answers for each one of the questions. RESULTS: Physicians from 38 (95 percent) of the 40 teams in the first and second divisions answered the questionnaires and 71.1 percent reported the occurrence of some type of dental injury during soccer practice, dental fractures (74.1 percent) and avulsions (59.3 percent) being the most prevalent ones. Regarding emergency conducts, approximately 50 percent answered that a successful replantation could be obtained in periods from 6 to 24 h after injury, and 27.8 percent were not able to answer this question. Regarding mouthguard use, 48.6 percent of the physicians did not know about mouthguards, and only 21.6 percent usually recommended their use by the soccer players. Among the physicians who do not recommend the use of mouthguards, 50 percent justified that it was not necessary. Almost 50 percent of the medical departments do not have a dentist as part of the health professional staff. CONCLUSIONS: It was possible to conclude that dental injuries are common during professional soccer practice and that there is a lack of information in the medical departments related to the emergency conducts and prevention of dental trauma.